Location & approach

By car

The campus of Dortmund University of Technology is located near the Dortmund West motorway junction, where the A45 Sauerland route crosses the B1/A40 Ruhr expressway. The Dortmund-Eichlinghofen exit on the A45 motorway leads to the South Campus, the Dortmund-Dorstfeld exit on the A40 motorway leads to the North Campus. The university is signposted at both exits.

By bus and train

The ‘Dortmund Universität’ S-Bahn station is located directly on the North Campus. From there, the S-Bahn line S1 runs every 20 or 30 minutes to Dortmund Central Station and in the opposite direction to Düsseldorf Central Station via Bochum, Essen and Duisburg. The university can also be reached by bus lines 445, 447 and 462. Timetable information can be found on the homepage of the Rhine-Ruhr transport association, and DSW21 also offers an interactive network map.

The H-Bahn

The H-Bahn is one of the landmarks of TU Dortmund University. Line 1 runs every 10 minutes between Dortmund Eichlinghofen and the Technology Centre via Campus South and Dortmund University S, while Line 2 runs every 5 minutes between Campus North and Campus South. It covers this route in two minutes.

By plane

From Dortmund Airport, you can take the AirportExpress to Dortmund Central Station in just over 20 minutes and from there take the S-Bahn to the university. Düsseldorf Airport, around 60 kilometers away, offers a wider range of international flight connections and can be reached directly by S-Bahn from the university station.

Plan of site & interactive map

The facilities of TU Dortmund University are spread over two campuses, the larger Campus North and the smaller Campus South. Additionally, some areas of the university are located in the adjacent "Technologiepark".

> Go to interactive map

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Location & approach

By car

The campus of Dortmund University of Technology is located near the Dortmund West motorway junction, where the A45 Sauerland route crosses the B1/A40 Ruhr expressway. The Dortmund-Eichlinghofen exit on the A45 motorway leads to the South Campus, the Dortmund-Dorstfeld exit on the A40 motorway leads to the North Campus. The university is signposted at both exits.

By bus and train

The ‘Dortmund Universität’ S-Bahn station is located directly on the North Campus. From there, the S-Bahn line S1 runs every 20 or 30 minutes to Dortmund Central Station and in the opposite direction to Düsseldorf Central Station via Bochum, Essen and Duisburg. The university can also be reached by bus lines 445, 447 and 462. Timetable information can be found on the homepage of the Rhine-Ruhr transport association, and DSW21 also offers an interactive network map.

The H-Bahn

The H-Bahn is one of the landmarks of TU Dortmund University. Line 1 runs every 10 minutes between Dortmund Eichlinghofen and the Technology Centre via Campus South and Dortmund University S, while Line 2 runs every 5 minutes between Campus North and Campus South. It covers this route in two minutes.

By plane

From Dortmund Airport, you can take the AirportExpress to Dortmund Central Station in just over 20 minutes and from there take the S-Bahn to the university. Düsseldorf Airport, around 60 kilometers away, offers a wider range of international flight connections and can be reached directly by S-Bahn from the university station.

Plan of site & interactive map

The facilities of TU Dortmund University are spread over two campuses, the larger Campus North and the smaller Campus South. Additionally, some areas of the university are located in the adjacent "Technologiepark".

> Go to interactive map

ServicePortal UniMail

Research Paper: Immobilization of the Amidohydrolase MxcM and Its Application for Biocatalytic Flow Synthesis of Pseudochelin A

01/18/2023

Immobilization of the Amidohydrolase MxcM

A new scientific article has been published.

Abstract:

The chemical synthesis of heterocycles typically requires elevated temperature and acid or base addition to form the desired product. Moreover, these reactions often involve hazardous reagents, which is why biocatalytic routes for heterocycle formation have gained increasing attention. In recent years, several enzymes belonging to the amidohydrolase superfamily have been identified to generate heterocycles via cyclocondensation reactions. Of particular interest is the amidohydrolase MxcM, which catalyzes the formation of an imidazoline moiety in the biosynthesis of the anti-inflammatory natural product pseudochelin A. In this study, we present a concept for the immobilization of this enzyme using a fused hexahistidine tag for fixation onto a solid, porous carrier. Notably, the immobilization improves the enzyme's tolerance to organic solvents. The immobilized MxcM exhibits a residual activity of 169% in the polar solvent acetonitrile compared to the free enzyme, and the storage stability in the presence of 20 vol% acetonitrile was ameliorated. In addition, an immobilized enzyme reactor (IMER) was designed that can be operated under flow conditions. The MxcM-IMER retains its biocatalytic activity and mechanic stability over the tested operation time. These results provide important insights for the integration of heterocycle-forming amidohydrolases in chemical processes.

The paper "Immobilization of the Amidohydrolase MxcM and Its Application for Biocatalytic Flow Synthesis of Pseudochelin A" can be found at https://www.mdpi.com/2073-4344/13/2/229. Enjoy reading.